To unshare particular USB port, please, follow the steps:
Switch to "Share local USB devices" tab on application's Toolbar.

Select USB device you want to share in USB devices tree.

Click "Unshare local USB device" Toolbar button or select "Share devices" -> "Unshare local USB devices" option from the Main menu.

Once the device is successfully unshared, the icon in USB devices tree will be changed to
and you will gain full control over the USB device.